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Cattle T4(Thyroxine) ELISA Kit orb1146811
https://www.biorbyt.com/cattle-t4thyroxine-elisa-kit-orb1146811.html
| Catalog Number | orb1146811 |
|---|---|
| Category | Assays and Kits |
| Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Thyroxine(T4) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Thyroxine(T4). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thyroxine(T4) in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Reactivity | Bovine |
| Range | 4.69-300 ng/mL |
| Sensitivity | 1.42 ng/mL |
| Protein Length | 2h |
| Alternative names | 3,5,3',5'-Tetraiodothyronine, L-Thyroxine, Levothyroxine Read less |
| Note | For research use only |
| Sample Types | serum, plasma and o |