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Human DCP(Des-gamma carboxyprothrombin) ELISA Kit orb1146819
https://www.biorbyt.com/human-dcpdes-gamma-carboxypro-orb1146819.html
| Catalog Number | orb1146819 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Des-gamma carboxyprothrombin(DCP). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Des-gamma carboxyprothrombin(DCP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Des-gamma carboxyprothrombin(DCP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Des-gamma carboxyprothrombin(DCP) in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Reactivity | Human |
| Range | 0.32-20 ng/mL |
| Sensitivity | 0.115 ng/mL |
| Protein Length | 3.5h |
| Alternative names | CAP4, FLICE, MACH, MCH5, Cysteinyl Aspartate Specific Proteinases 8, Apoptotic cysteine protease, FADD-homologous ICE/ced-3-like protease, MORT1-associated ced-3 homolog Read less |
| Note | For research use only |
| Sample Types | Tissue homogenates, cell lysates and other biological fluids |
| Expiration Date | Please enquire. |