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Human NKX2-5(NK2 Homeobox 5) ELISA Kit orb1817355 https://www.biorbyt.com/human-nkx2-5nk2-homeobox-5-e-orb1817355.html
| Catalog Number | orb1817355 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NKX2-5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NKX2-5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NKX2-5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NKX2-5 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Reactivity | Human |
| Range | 78.13-5000 pg/mL |
| UniProt ID | P52952 |
| Sensitivity | 21.3 pg/mL |
| Alternative names | Cardiac-specific homeobox; Homeobox protein CSX; H Read more... |
| Note | For research use only |
| Sample Types | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Expiration Date | Please enquire. |