EasyStep Human PGII(Pepsinogen II) ELISA Kit orb1817399
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说明书 orb1817399.pdf
Catalog Number | orb1817399 |
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Category | Assays and Kits |
Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PGII, and the Human PGII standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human PGII. After TMB substrate solution is added, only those wells that contain Human PGII and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PGII in the samples is then determined by comparing the OD of the samples to the standard curve. |
Reactivity | Human |
Range | 0.16-10 ng/mL |
Sensitivity | 7 pg/mL |
Alternative names | PGII Read more... |
Note | For research use only |
Sample Types | Serum, plasma |
Expiration Date | Please enquire. |