【Biorbyt 技术】如何选择合适的流式抗体？

什么是流式细胞术？

流式细胞术（Flow Cytometry，FC）是一种基于荧光的，在液流系统中快速测定单个细胞或细胞器的生物学性质，例如细胞群计数和蛋白丰度等。流式细胞术结合细胞分选仪还可以进一步把特定的细胞或细胞器从群体中加以分类收集的技术,该过程称为荧光激活细胞分选法 (FACS)。流式细胞技术在细胞生物学、细胞凋亡研究和免疫学方面起着非常重要的作用。

流式细胞仪如何工作？

在流式细胞术中使用的仪器称为流式细胞分析仪，通常缩写为“细胞分析仪”。流式细胞分析仪由一个用于进行细胞处理的流体系统和一个包括数据采集信号检测器和处理器的光学系统组成。

关于流式细胞仪更详细的工作原理，点击以下视频了解。

流式细胞实验中如何选择合适的抗体？

在流式细胞实验过程中，荧光抗体对单细胞悬液的标记效果直接影响着实验的数据质量。

随着流式细胞术的日益发展，流式抗体及荧光标记产品的种类、数量也在不断增多，加上多色分析实验方案需求的增长，逐渐产生了抗体难选择、荧光难搭配等问题，如何选择合适的流式抗体，也成为了流式技术是否能成功的关键因素之一。

Biorbyt 在本篇文章中就来聊聊流式抗体选择的小技巧。

1）确定抗体基本信息

确定所需检测的指标，目的细胞的特异性表面标记或胞内标记；
根据检测样本的种属选择可适用的抗体；
根据抗体的说明，明确是否可以用于流式实验。

2）确定所使用仪器的参数配置

激光器：不同的流式细胞仪激光器不同，常见的有405nm，488nm和635nm，需要根据具体的仪器进行确定。
滤光片：检测通道的多少，决定最多可以同时检测几个指标。荧光素的发射波长与滤光片有关，所选择荧光素要在仪器的可检测范围内。
仪器：相同的荧光在不同的仪器上检测通道可能不同，需要参照仪器的说明。

3）荧光素的选择

抗原表达弱或分群不明显的建议选择强荧光，如PE、APC。
抗原表达强或分群明显的建议选择最常用的弱荧光例如FITC。
常用荧光强弱排序程度为：PE>APC>PE-cy5Cy5>PercCPp-Cycy5.5>FITC

4）多色荧光搭配

同时检测几个指标时，就需要多个荧光标记进行搭配。流式抗体荧光标记搭配主要由3个因素决定的：流式细胞仪能检测多少个通道、需要同时检测检测多少个指标、厂家的抗体有多少种荧光标记。
注意所使用的仪器有哪些激光，每个激光检测通道只能选择1种荧光素，各通道之间的荧光素可以随意搭配，检测通道不能冲突。
如果需要做多个指标，而流式细胞仪能检测的通道不够，科研将指标归成2类，再将样本分成2份，分别进行检测。
所选各种荧光素光谱的重叠应当尽量减少，否则将会导致较大的补偿。例如，PE-Cy5与APC同时标记，将会导致补偿过度，需换成PE-Cy5.5或PerCP-Cy5.5。

5）同型对照抗体的选择

同型对照，是用于消除由于抗体非特异性结合到细胞表面而产生的背景染色，类似于阴性对照，是流式细胞实验不可或缺的一项。
同型对照是使用与一抗相同种属来源、相同亚型及亚链、相同荧光标记的免疫球蛋白，也要求使用相同剂量。如一抗是抗human CD3-FITC(mouse IgG1)，同型对照则为Mouse IgG1-FITC.
如果是纯化的一抗加荧光标记的二抗，那么应该选择与一抗相对应的同型对照抗体，如纯化的CD3+PE标记的二抗，则对照为纯化的同型对照+ PE标记的二抗。
有时候同型对照的表达会比抗体的表达高，首先检查同型对照的抗体是否加错类型、剂量等。其次，因为有些标志在细胞的表面或者胞内表达不高，而跟其类似的抗原非常多，那么同型对照非特异性的结合会超过抗体的特异性，所以会造成这种现象。这个时候推荐使用其他阴性对照，如空白对照等。

6）直接法与间接法染色的选择

直接法：即使用直接偶联抗体，可直接多重分析来自同一宿主的抗体。由于孵育和洗涤步骤更少，因此实验步骤更快，并且能够在一项检测中使用许多不同的抗体，从而能够同时读取大量终点。
间接法：即使用非偶联一抗 + 偶联二抗，可改善以低丰度存在的靶标的检测，同时由于二次放大可提供更高的灵敏度（多个二抗结合一个一抗）。当直接偶联物尚未获得时，有助于一抗的初始验证。

扫码获得英国biorbyt热销流式抗体的详细产品信息清单

Biorbyt 可以提供各种研究领域蛋白指标的流式细胞检测抗体，以下是近期热销产品列表，欢迎咨询！

Catalog No.	Product Name	Applications
orb500265	Smad3 antibody	ICC,FC,WB,IHC-P
orb526646	Akt1 (phospho-Ser473) antibody	ICC,FC,WB,IHC-P
orb559171	YAP1 (phospho-Ser127) antibody	FC,WB
orb612211	TNFR2 antibody	ICC,FC,WB,IHC-P
orb704335	Wnt2b antibody	ICC,FC,WB,IHC-P
orb704345	LYVE1 antibody	ICC,FC,IHC-P
orb704355	Alpha Smooth Muscle Actin antibody	ICC,FC,WB,IHC-P
orb499643	Beta-Actin antibody	FC,WB,IHC-P
orb525868	CD19 antibody	FC
orb182468	CD163 antibody	ICC,FC,WB,IHC-P,IF
orb182470	CD4 antibody	ICC,FC,WB,IHC-P
orb182473	Cryopyrin antibody	FC
orb182660	Contactin 1 antibody	FC
orb182936	Cardiac Troponin T antibody	ICC,FC,IHC-P
orb183142	DNAH5 antibody	FC
orb183268	beta Defensin 3 antibody	FC,IF
orb183495	NeuN antibody	ICC,FC,IHC-P,IF
orb183601	FAM38A antibody	ICC,FC,IHC-P,IF
orb184006	HPV16 E7 antibody	FC,WB
orb184283	CD41 antibody	FC
orb184816	KAAG1 antibody	FC
orb184818	KAP1 (phospho-Ser473) antibody	FC,IHC-P
orb184851	LAPTM5 antibody	FC
orb185667	Occludin antibody	FC,WB
orb186433	Tropomyosin (phospho-Tyr490) antibody	FC,WB
orb186439	TNNI3 antibody	FC,WB,IHC-P
orb186927	CHRAC1 antibody (FITC)	FC
orb187072	CHI3L1 antibody (FITC)	FC,IF
orb187180	Claudin 5 antibody (FITC)	FC
orb187808	NeuN antibody (FITC)	ICC,FC,IF
orb187914	FAM38A antibody (FITC)	ICC,FC,IF
orb188680	Insulin Receptor (phospho-Tyr999) antibody (FITC)	FC,IF
orb189165	LAPTM5 antibody (FITC)	FC
orb189951	Nephrin antibody (FITC)	FC
orb312158	Activated Notch1 antibody	FC,WB,IHC-P
orb312168	ALPPL2 antibody	FC,IHC-P
orb312182	CLEC2 antibody	FC
orb312217	DRP1 (phospho-Ser616) antibody	FC,IHC-P
orb312227	alpha Elastin antibody	FC,WB,IHC-P
orb312249	ganglioside GM1 antibody	FC,IHC-P
orb312397	NEP1-40 antibody	FC
orb312399	NFKB p65 antibody	FC,WB,IHC-P
orb312400	NFKB p65 antibody	FC,IHC-P
orb312509	PKM2 (phospho-Tyr148) antibody	FC
orb312830	PCDH7 antibody	FC,WB
orb312831	beta 1 Adrenergic Receptor antibody	FC,WB
orb313249	TREM2 antibody	FC,WB,IHC-P
orb317567	CD19 antibody	FC,IHC-P
orb317598	CD68 antibody	FC,WB,IF
orb317686	NFKB p65 antibody	ICC,FC,WB,IHC-P
orb317701	PPARGC1A antibody	FC,WB,IHC-P
orb317880	SLC15A3 antibody	FC
orb483321	HB9/HLXB9 antibody (PE)	ICC,FC,IF
orb483598	PPAR Gamma antibody (PE)	FC,IF
orb10037	ACTA2 antibody	FC,WB
orb10039	AD7C-NTP antibody	FC,WB,IHC-P,ELISA
orb10059	AEBP1 antibody	FC,IHC-P
orb10068	F-Actin antibody	FC,WB,IHC-P
orb10101	Annexin V antibody	FC,WB,ELISA
orb10124	Aquaporin 3 antibody	FC,IHC-P
orb10143	MASH1/Achaete-scute homolog 1 antibody	ICC,FC,WB,IHC-P
orb10158	ATP7A antibody	FC,IHC-P
orb10181	BDNF antibody	FC,WB,IHC-P
orb10186	FGF2 antibody	FC,WB,IHC-P
orb10277	Interferon gamma antibody	FC,IHC-P,IF
orb10289	Syndecan 1 antibody	FC,WB,IHC-P
orb10295	CD28 antibody	FC,IHC-P
orb10305	CXCR4 antibody	FC,WB,IHC-P
orb10309	CD24 antibody	FC,WB
orb10313	CD3 antibody	FC,WB,IF
orb10371	CEBP beta antibody	FC,WB,IHC-P
orb10380	Chat antibody	FC,WB,IHC-P,IHC,IF
orb10393	c-Jun antibody	FC,WB,IHC-P
orb10399	pan Cytokeratin antibody	ICC,FC,WB,IHC-P,IF
orb10496	Cyclin D1 antibody	ICC,FC,WB,IHC-P
orb10514	Dopamine D1 receptor antibody	FC,WB,IHC-P
orb10573	HPV16 E7 antibody	FC
orb10581	EGF antibody	ICC,FC
orb10670	FOXP3 antibody	FC,IHC-P
orb10681	GAD67 antibody	FC,WB,IHC-P
orb10684	DDIT3 antibody	ICC,FC,WB
orb10726	GLUT2 antibody	FC,WB,IHC-P
orb10740	GPR30 antibody	ICC,FC,WB,IHC-P
orb10837	HPV16 E6 protein antibody	FC
orb10839	HPV16-E7/E7 antibody	FC
orb10855	TRT antibody	ICC,FC,WB,IHC-P
orb10863	AIF1 antibody	FC,WB,IHC-P,ELISA
orb10917	CD127 antibody	FC,WB
orb10927	Integrin alpha 5 beta 3 antibody	FC,WB,IHC-P
orb10952	ATP1B2 antibody	FC,WB,IHC-P
orb10973	OxLDL antibody	FC,IHC-P
orb10982	GPR49 antibody	FC,WB,IHC-P
orb11009	CD11b antibody	FC,IHC-P
orb11040	Mfn1 antibody	FC
orb101049	MMP-2 antibody	ICC,FC,WB
orb11082	Mesothelin antibody	FC,WB,ELISA
orb11118	NFkB p65 antibody	ICC,FC,WB,IHC-P,IF
orb11125	NGF Receptor antibody	FC,WB
orb11163	GRIN2D antibody	FC
orb11184	OCT4 antibody	FC,WB
orb11190	RANKL antibody	FC,WB,IHC-P
orb11192	Osteopontin antibody	FC,WB,IHC-P,IF
orb11201	CDKN2A antibody	FC,IHC-P
orb11203	p21 antibody	ICC,FC,IHC-P
orb11225	PAF Receptor antibody	FC,IHC-P
orb11256	PDGF Receptor alpha antibody	FC,IHC-P
orb11372	SIRT1 antibody	FC,WB,IHC-P
orb11384	Smad2 antibody	FC,WB,IHC-P
orb11407	Surfactant Protein D antibody	FC,IHC-P
orb106147	STAT3 (phospho-Tyr705) antibody	FC,WB,IHC-P
orb11470	TGF beta 3 antibody	FC,WB,IHC-P
orb11472	TGF beta Receptor I antibody	FC,WB,IHC-P,IF
orb11489	TLR4 antibody	ICC,FC,WB,IHC-P
orb11501	TPO antibody	FC
orb11506	TNF Receptor II antibody	FC,WB
orb11522	TRX antibody	ICC,FC,WB,IHC-P
orb11559	Vimentin antibody	ICC,FC,WB,IHC-P
orb11580	XBP1 antibody	ICC,FC,WB
orb15046	F-Actin antibody (FITC)	FC
orb15056	Adiponectin Receptor 2 antibody (FITC)	FC,IF
orb15062	Adenosine Receptor A3 antibody (FITC)	FC,IF
orb15065	ADRB2 antibody (FITC)	FC,IF
orb15126	Aquaporin 9 antibody (FITC)	FC
orb15268	CCR7 antibody (FITC)	ICC,FC,IF
orb15344	CDK4 antibody (FITC)	ICC,FC,IF
orb15391	pan Cytokeratin antibody (FITC)	ICC,FC,IF
orb15501	Desmin antibody (FITC)	FC
orb15659	GFAP antibody (FITC)	FC,IF
orb15756	HIF2 alpha antibody (FITC)	FC,IF
orb15948	LC3 antibody (FITC)	FC,IF
orb15949	MAP2 antibody (FITC)	FC,IF
orb15955	MBP antibody (FITC)	FC,IF
orb16133	CDKN2A antibody (FITC)	FC,IF
orb16252	Profilin 1 antibody (FITC)	FC,IF
orb16404	TGF beta Receptor 1 antibody (FITC)	FC,IF
orb16446	TrkB antibody (FITC)	FC,IF
orb16451	TRX antibody (FITC)	ICC,FC,IF
orb16490	Vimentin antibody (FITC)	ICC,FC,IF
orb13228	5HT4 Receptor antibody	FC,WB,IHC-P,IF
orb13306	CD226/DNAM-1 antibody	FC,WB
orb13313	SLAMF9 antibody	FC,WB
orb13317	CD9 antibody	FC,WB
orb13342	Cytokeratin 7 antibody	ICC,FC,WB,IHC-P
orb13385	EAR1 antibody	FC,WB,IHC-P
orb13390	EIF4E1 (phospho-Ser209) antibody	FC,WB,IHC-P
orb13426	CXCL13 antibody	FC,WB,IHC-P,IF
orb13496	IL15 antibody	ICC,FC,IHC-P
orb13514	Integrin alpha 1 antibody	FC,WB
orb13515	Integrin Alpha V + Beta 1 antibody	FC,WB
orb13540	LEF1 antibody	FC,WB
orb13563	MCP1 antibody	FC,IHC-P
orb13605	Neuritin antibody	FC,WB
orb13641	PD1 antibody	FC,WB,IHC-P
orb13645	PERK antibody	FC,WB,IHC-P
orb13652	Polycystin 1 antibody	FC
orb13678	ROS antibody	FC,WB
orb13722	TLR1 antibody	FC
orb13739	UCP1 antibody	FC,WB
orb13740	UCP2 antibody	ICC,FC,WB,IHC-P
orb13821	BAFF antibody (FITC)	FC,IF
orb13894	SMMHC antibody (FITC)	FC
orb13919	CYP3A4 antibody (FITC)	FC,IF
orb13948	EAR1 antibody (FITC)	FC,IF
orb14085	Klf4 antibody (FITC)	FC,IF
orb14198	PD1 antibody (FITC)	FC,IF
orb14246	SREBP2 antibody (FITC)	ICC,FC,IF
orb15031	PPAR alpha antibody	FC,WB,IHC-P
orb5645	4EBP1 (phospho-Thr37/46) antibody	ICC,FC
orb4401	BACH1 antibody	FC,WB,IHC-P
orb4691	BMI-1 antibody	FC,WB
orb5888	CLEC2D antibody	FC
orb5872	CK19 antibody	FC,WB,IHC-P
orb5845	PBR antibody	FC,IHC-P
orb5211	FAK (phospho-Tyr576/577) antibody	FC,WB,IHC-P
orb6097	Glucose 6 phosphatase alpha antibody	FC,WB,IHC-P
orb6078	GCN2 (phospho-Thr899) antibody	FC
orb5384	TIM1 antibody	FC
orb6159	Tri Methyl-Histone H3 (K27) antibody	FC
orb5482	HSP27 (phospho-Ser82) antibody	FC,IHC-P
orb5499	IFNAR1 antibody	ICC,FC,WB,IHC-P
orb99035	LRP1 (phospho-Ser452) antibody	FC,IHC-P
orb6173	Lrp2 antibody	FC,IHC-P
orb5651	Btk (phospho-Tyr223) antibody	FC,IHC-P
orb6019	NR1D1 antibody	FC,WB,IHC-P
orb6191	HMGCR (phospho-Ser872) antibody	FC,WB,IHC-P
orb6232	IRF7 antibody	FC,IHC-P
orb6328	CD91 antibody	FC,IHC-P
orb6344	CD103 antibody	FC,WB
orb6419	MEF2D (phospho-Ser444) antibody	FC,WB
orb6424	MLK3 (phospho-Thr277/Ser281) antibody	FC,IHC-P
orb6519	eNOS (phospho-Ser1177) antibody	FC,WB
orb6544	Nrf2 (phospho-Ser40) antibody	ICC,FC,WB,IHC-P
orb6571	CDKN1A (phospho-Ser130) antibody	FC,IHC-P
orb6786	PKR (phospho-Thr446/451) antibody	ICC,FC,WB,IHC-P
orb6979	Smad2 (phospho-Ser465/467) antibody	ICC,FC,WB,IHC-P
orb7067	Tau protein (phospho-Thr205) antibody	FC,WB
orb7086	TGF Beta 1(CT) antibody	FC,WB,IHC-P
orb7087	TGF Beta 1 antibody	FC,WB,IHC-P,IF
orb7128	Tubulin alpha 6 antibody	FC,WB,IHC-P
orb7143	CD21 antibody	FC,WB
orb8720	CK19 antibody (FITC)	FC,IF
orb8717	CK10 antibody (FITC)	ICC,FC,IF
orb7831	CREM antibody (FITC)	ICC,FC,IF
orb8063	FAK (phospho-Tyr925) antibody (FITC)	FC,IF
orb8550	Vitamin D Binding protein antibody (FITC)	FC
orb7235	SOX9 antibody (FITC)	FC,IF
orb9072	IRF3 antibody (FITC)	FC,IF
orb9097	Jak1 (phospho-Tyr1022/1023) antibody (FITC)	FC
orb9176	CD91 antibody (FITC)	FC,IF
orb9301	mTOR (phospho-Ser2481) antibody (FITC)	ICC,FC,IF
orb9314	MyoD1 (phospho-Ser200) antibody (FITC)	FC
orb9715	RECK antibody (FITC)	FC,IF
orb9766	Rabbit Anti-Sca1 (FITC)	FC,IF
orb9848	SP-C antibody (FITC)	FC
orb9909	Tau protein (phospho-Thr231) antibody (FITC)	FC
orb9944	TLR6 antibody (FITC)	FC,IF
orb6174	Collagen IV antibody	FC,IHC-P
orb11061	MMP2 antibody	FC,WB,IHC-P
orb101887	ACPT antibody	FC
orb101745	IL1 beta antibody	FC,WB,IHC-P
orb101675	TdT antibody	FC,IHC-P
orb101668	CD16 antibody	FC,WB,IHC-P
orb101636	FAPA antibody	FC,IHC-P
orb101303	HAS antibody	FC,WB
orb100708	MFSD2A antibody	FC,WB,IHC-P
orb100539	Bone Alkaline Phosphatase antibody	FC
orb100329	TNFR1 antibody	FC,WB
orb100289	Integrin Alpha V/Beta 6 antibody	FC,WB
orb100194	EDG3 antibody	FC,WB,IHC-P
orb100121	PCSK9 antibody	ICC,FC,WB,IHC-P
orb102042	ADORA2B antibody (FITC)	ICC,FC,IF
orb102419	CYP2E1 antibody (FITC)	FC,IF
orb102500	EDIL3 antibody (FITC)	FC
orb102575	FAPA antibody (FITC)	FC,IF
orb102620	Frizzled 1 antibody (FITC)	FC,IF
orb102715	HDLBP antibody (FITC)	FC
orb102779	ICAM4 antibody (FITC)	FC,IF
orb103060	Myosin VIIa antibody (FITC)	FC,IF
orb103138	Nucleoporin p62 antibody (FITC)	FC,IF
orb103247	Plzf antibody (FITC)	FC,IF
orb1117	Geminin antibody	FC,IHC-P
orb1179	CD45 antibody	FC,WB
orb1255	UBE2Q1 antibody	FC
orb1265	Arginase 1 antibody	FC,IHC-P
orb1532	Nitrotyrosine antibody	FC,WB
orb1777	Dlx1 antibody	FC
orb1860	pIRF3 antibody	FC,WB,IHC-P
orb1868	CD71 antibody	FC,WB,IHC-P
orb1869	FOXN1 antibody	FC,WB,IHC-P
orb1926	IGSF9 antibody	FC
orb2059	MSX1 antibody	FC,WB
orb2101	PPAR Gamma antibody	FC,WB,IHC-P
orb2250	TRAP antibody	FC,WB
orb2664	FL 393 antibody	ICC,FC,WB,IHC-P,ELISA
orb2733	RNF123 antibody	FC,WB,IHC-P
orb3009	CXorf21 antibody	FC
orb3644	Neutrophil Elastase antibody (FITC)	FC
orb3898	CD71 antibody (FITC)	FC
orb3910	pTrkA antibody (FITC)	FC,IF
orb499146	FCGRT antibody (PE)	ICC,FC,IF
orb499317	Dopamine D2 receptor antibody (PE)	FC,IF
orb499522	CD206 antibody (PE)	FC
orb499560	Cryopyrin antibody (PE)	FC,IF
orb499606	BTN2A1 antibody	FC
orb499639	IL-1R1 antibody	ICC,FC,WB,IHC-P
orb499655	PPAR Alpha antibody	FC,WB,IHC-P
orb499679	SOD2 antibody	FC,WB,IHC-P
orb499694	AD7c-NTP antibody	FC
orb499738	CD90 antibody	FC,WB
orb500026	PERK (phospho-Thr980) antibody	ICC,FC,IHC-P
orb500678	LRRK2 antibody	FC,IHC-P
orb500732	NKG2D antibody	FC,WB
orb500740	SOX10 antibody	FC,IHC-P
orb500812	Ki-67 antibody	FC,WB,IHC-P
orb500824	GPR30 antibody	ICC,FC,WB,IHC-P
orb500829	GFAP antibody	FC,WB,IHC-P,IF
orb500922	CD11c antibody	FC
orb500942	CD11c antibody	FC,WB
orb501007	Cox2 antibody	FC,WB,IHC-P
orb502710	DRP1 (phospho-Ser616) antibody (FITC)	ICC,FC,IF
orb504819	p70 S6 Kinase Beta (phospho-Thr389) antibody (PE)	FC
orb505121	BNIP3 (Phospho-Ser95) antibody (PE)	FC
orb505547	EGFR (phospho-Tyr1068) antibody (PE)	ICC,FC,IF
orb505631	FoxO1 (phospho-Ser256) antibody (PE)	FC,IF
orb505720	REV-ERB alpha (phospho-Ser55/59) antibody (PE)	FC
orb505918	FAK (phospho-Tyr925) antibody (PE)	FC,IF
orb505973	RUNX2 (phospho-Ser451) antibody (PE)	FC
orb506137	Beta-Catenin (phospho-Ser552) antibody	FC
orb506147	PERK (phospho-Thr980) antibody	FC,WB,IHC-P
orb155740	ASCL2 antibody	ICC,FC
orb155807	BCL2 (Phospho-Ser87) antibody	FC,IHC-P
orb156292	CCL26 antibody	FC,IHC-P
orb156359	CHRM4 antibody	FC,WB
orb156402	CLIC6 antibody	FC
orb156486	CTLA4 antibody	FC
orb156726	EMR1 antibody	FC,WB
orb156854	FCGRT antibody	FC,WB
orb156945	Fragilis antibody	FC
orb156973	FXR antibody	FC,WB
orb157177	Glucocorticoid Receptor beta antibody	FC,WB,IHC-P
orb1184460	CD69 antibody (FITC)	FC
orb157302	GPR110 antibody	FC,WB
orb157428	HAPLN3 antibody	FC
orb157435	HB9 antibody	ICC,FC,WB
orb1184523	IL-4 antibody (APC/Cy7)	FC
orb157743	Keratan Sulfate antibody	FC,WB
orb157860	MCT1 antibody	FC,WB,IHC-P
orb157917	Musashi 1 antibody	ICC,FC,IHC-P
orb158462	SOX4 antibody	FC,WB,IHC-P
orb158576	TBR2 antibody	FC,IHC-P
orb158598	THRB1 antibody	FC,WB,IHC-P
orb158607	TIFA antibody	FC,IHC-P
orb158655	TRPV6 antibody	FC,WB
orb158714	Vimentin antibody	ICC,FC,WB,IF
orb155585	ACTA2 antibody	FC,WB,IHC-P
orb484364	Frizzled 1 antibody (PE)	FC,IF
orb484458	Frizzled 5 antibody (PE)	FC,IF
orb484899	PDE5A antibody (PE)	FC,IF
orb485167	CD170 antibody (PE)	FC,IF
orb485179	CLEC2D antibody (PE)	FC,IF
orb485226	Histone 2AX antibody (PE)	ICC,FC,IF
orb485585	MC1 Receptor antibody (PE)	FC,IF
orb485741	MADH7 antibody (PE)	ICC,FC,IF
orb485757	Synaptophysin antibody (PE)	FC,IF
orb485787	PDGF A antibody (PE)	FC,IF
orb485798	Substance P Receptor antibody (PE)	FC,IF
orb485838	IL1 beta antibody (PE)	FC,IF
orb485900	FGFR2 antibody (PE)	ICC,FC,IF
orb485929	Desmin antibody (PE)	FC
orb485949	CD10 antibody (PE)	ICC,FC,IF
orb485955	CD20 antibody (PE)	FC
orb485978	GLUT1 antibody (PE)	FC,IF
orb487111	TRPV6 antibody (PE)	ICC,FC,IF
orb487192	TRPV5 antibody (PE)	ICC,FC,IF
orb487237	Calcitonin receptor antibody (PE)	FC
orb488732	MSGN1 antibody (PE)	FC
orb490178	ALPPL2 antibody (PE)	ICC,FC,IF
orb490863	NG2 antibody (PE)	FC
orb491176	SOCS3 antibody (PE)	FC,IF
orb491183	Nurr1 antibody (PE)	FC,IF
orb491286	BTN2A1 antibody (PE)	ICC,FC,IF
orb491300	Synaptopodin antibody (PE)	FC
orb491535	PCDH7 antibody (PE)	ICC,FC,IF
orb492352	METRNL antibody (PE)	FC
orb493558	FAM105B antibody (PE)	FC
orb493841	Galectin-1 antibody (PE)	FC,IF
orb493916	LPA2 antibody (PE)	ICC,FC,IF
orb494806	Fragilis/IP15 antibody (PE)	ICC,FC,IF
orb495013	METRN antibody (PE)	FC
orb495075	5-Lipoxygenase antibody (PE)	FC,IF
orb495188	GFAP antibody (PE)	FC,IF
orb495270	p21 antibody (PE)	ICC,FC,IF
orb495275	Vip Receptor II antibody (PE)	FC,IF
orb495322	STAT5 antibody (PE)	ICC,FC,IF
orb495392	CCR7 antibody (PE)	ICC,FC,IF
orb495404	ZO-1 antibody (PE)	FC,IF
orb495416	NRF1 antibody (PE)	ICC,FC,IF
orb495426	Granzyme B antibody (PE)	FC,IF
orb495512	CD33 antibody (PE)	FC
orb495598	FOXJ1 antibody (PE)	FC,IF
orb495625	MKP-2/DUSP4 antibody (PE)	FC,IF
orb495793	CK19 antibody (PE)	FC,IF
orb495861	Myosin light chain antibody (PE)	FC
orb496312	Neuroligin 1 antibody (PE)	FC
orb496784	Biglycan antibody (PE)	FC
orb497141	RAG1 antibody (PE)	FC,IF
orb498451	SOX4 antibody (PE)	ICC,FC,IF
orb499007	SULF1 antibody (PE)	FC,IF
orb499027	Rabbit Anti-Sca1 (PE)	FC,IF
orb499041	HER2 antibody (PE)	FC,IF
orb499090	PDGF B antibody (PE)	FC,IF
orb499099	Mesothelin antibody (PE)	FC
orb499101	N Cadherin antibody (PE)	ICC,FC,IF
orb463034	SLC34A3 antibody (FITC)	FC
orb463588	IL-1R1 antibody (FITC)	ICC,FC,IF
orb463625	CD19 antibody (FITC)	FC
orb464231	Nurr1 antibody (FITC)	FC,IF
orb464334	BTN2A1 antibody (FITC)	ICC,FC,IF
orb464413	PCDH7 antibody (FITC)	ICC,FC,IF
orb464951	CD200R antibody (FITC)	ICC,FC,IF
orb464992	DAP12 antibody (FITC)	FC,IF
orb465963	CD147 antibody (FITC)	FC,IF
orb466005	MADH7 antibody (FITC)	ICC,FC,IF
orb466068	IL1 Receptor 2 antibody (FITC)	FC,IF
orb466729	EN1/Engrailed 1 antibody (FITC)	ICC,FC,IF
orb466849	iNOS antibody (FITC)	ICC,FC,IF
orb466860	CD11c antibody (FITC)	FC
orb466967	beta 1 Adrenergic Receptor antibody (FITC)	FC,IF
orb124537	CD69 antibody (PE)	FC,IF
orb124560	Cox2 antibody (PE)	FC,IF
orb124566	CXCL10 antibody (PE)	FC,IF
orb124596	EAR1 antibody (PE)	FC,IF
orb124701	Integrin alpha 5 beta 3 antibody (PE)	FC,IF
orb124805	PAF Receptor antibody (PE)	FC,IF
orb461433	Cathepsin B antibody (FITC)	ICC,FC,IF
orb461506	Nogo A antibody (FITC)	FC,IF
orb461618	IGF1R antibody (FITC)	ICC,FC,IF
orb462375	GPR110 antibody (FITC)	ICC,FC,IF
orb462503	GPCR EX33 antibody (FITC)	ICC,FC,IF
orb462617	TRPV6 antibody (FITC)	ICC,FC,IF
orb462681	PLAC8 antibody (FITC)	FC
orb222130	MDR1 antibody (FITC)	ICC,FC,IF
orb526360	CD14 antibody	FC
orb526772	SLC25A18 antibody	FC,WB,IHC-P
orb544598	NCR1 antibody	FC,WB,IHC-P
orb612166	PAX6 antibody	FC,WB,IHC-P
orb704537	IRS1 (phospho-Tyr1229) antibody	ICC,FC,IHC-P
orb868319	Rabbit Anti-Mouse IgG H&L/FITC antibody	FC
orb868396	Mouse Anti-Human IgM antibody (Cy5)	FC
orb868531	Goat Anti-Rat IgG H&L antibody (Cy5)	FC
orb868595	Goat Anti-Rabbit IgG H&L antibody (PE-Cy5)	FC
orb868831	Rabbit Anti-Bovine IgM antibody (FITC)	FC
orb871516	Anti-IL-1 Beta/PE-Cy5	FC
orb878042	Anti-MARCH9/PE-Cy7	FC
orb879446	Anti-IRAK1/PE-Cy7	ICC,FC
orb880682	Anti-phospho-HNF4 (Ser304)/PE-Cy7	FC
orb881904	Anti-PPAR Gamma/PE-Cy5.5	FC
orb891238	Anti-FAPA/Cy5	FC,IF
orb891331	Anti-Neuritin/PE-Cy5	FC
orb891519	Anti-Polycystin 1/PE-Cy5	ICC,FC
orb892191	Anti-Ferritin Heavy Chain/FTH1/PE-Cy7	ICC,FC
orb899278	CD11b/PE-Cy5 antibody	FC
orb899559	Anti-Caveolin-3/Cy5	FC,IF
orb899751	Anti-PXR/PE-Cy5.5	FC
orb901017	Anti-CD1d/PE-Cy5.5	FC
orb902613	Phospho-PERK(Thr980)/PE-Cy7 antibody	FC
orb905794	Anti-CD170/PE-Cy5.5	FC
orb909518	Anti-CCR10/PE-Cy7	FC
orb909815	Anti-Thioredoxin/Cy5	ICC,FC,IF
orb910370	Profilin 1/PE-Cy5.5 antibody	FC
orb911309	Anti-BRG-1/PE-Cy7	FC
orb913013	Anti-KRAS/Cy5	FC,IF
orb913028	Anti-IL12/PE-Cy5.5	FC
orb913704	Anti-BLBP/Cy5	FC,IF
orb913879	Anti-GFAP/Cy5	FC,IF
orb914484	Anti-Nestin/PE-Cy7	ICC,FC
orb915495	Anti-CD19/Cy5	FC,IF
orb915599	Anti-alpha smooth muscle Actin/PE-Cy3	FC
orb917495	Anti-F-Actin/PE-Cy7	FC
orb917499	Anti-RAGE/PE-Cy7	FC
orb917649	Anti-GLUT1/PE-Cy5.5	FC
orb941999	Anti-NeuN/PE-Cy7	ICC,FC
orb959894	Anti-GFAP/PE-Cy5.5	ICC,FC
orb959955	Anti-NFKB p65(acetyl-K310)/PE-Cy5	FC
orb967450	Phospho-Smad2 (Ser250)/PE-Cy5.5	FC
orb967820	Anti-VWF/PE-Cy7	FC
orb967825	Anti-Synaptopodin/PE-Cy7	FC
orb973383	Anti-PXR/PE-Cy5	FC
orb979674	Anti-ZNF683/Cy3	FC
orb982588	Anti-Sca1/Cy3	FC,IF
orb991834	Anti-SP-C/APC	FC
orb993436	Anti-Bone Alkaline Phosphatase/APC	FC,IF
orb993553	Anti-CD16/APC	FC,IF
orb993611	Anti-Calreticulin/APC	FC,IF
orb994742	Anti-SREBP2/APC	ICC,FC,IF
orb994938	Anti-Dopamine D5 receptor/APC	FC,IF
orb995017	Anti-Claudin 1/APC	FC
orb995082	Anti-CXCL10/IP10/APC	FC,IF
orb995560	Anti-CD20/APC	FC
orb998027	Anti-CD44v3/APC	ICC,FC,IF
orb1000842	Anti-Phospho-MYPT1(Thr696)/APC	ICC,FC,IF
orb1001893	Anti-VNN1/APC	ICC,FC,IF
orb1004685	Anti-FCGRT/APC	ICC,FC,IF
orb1005088	Anti-Fragilis/APC	ICC,FC,IF
orb1005524	Anti-Cytokeratin 4/APC	FC,IF
orb1005708	Anti-Nogo-A/APC	FC,IF
orb1005735	Anti-PDGFRA/APC	FC,IF
orb1005736	Anti-PDGF-B/APC	FC,IF
orb1005799	Anti-TGF beta Receptor I/APC	FC,IF
orb1006424	Anti-EGFRvIII/APC	ICC,FC,IF
orb1006496	Anti-CLEC2D/APC	FC,IF
orb1007330	Anti-Phospho-mTOR (Thr2446) /APC	FC,IF
orb1007509	Anti-SOX9/APC	FC,IF
orb1007931	Anti-CKAP4/APC	FC,IF
orb1009483	Anti-PAX6/APC	ICC,FC,IF
orb1009572	Anti-TPOR/APC	FC
orb1046500	Anti-Amphiregulin/Cy5.5	FC
orb1048324	Anti-Phospho-Histone H2A.X (Ser139)/Cy7	ICC,FC,IF
orb1063819	EP4 antibody	FC,WB
orb1572137	Phospho-HSD17B13 (Ser33) Antibody	ICC,FC,WB
orb1577230	VEGFR3 Antibody (Cy5)	ICC,FC
orb1583206	E cadherin Antibody (PerCP)	ICC,FC
orb1584383	RUNX1 Antibody (BF555)	FC
orb1588962	ganglioside GM1 Antibody (PerCP)	FC
orb1589955	14-3-3 Antibody (BF750)	FC
orb1589956	14-3-3 Antibody (BF680)	FC
orb1589957	14-3-3 Antibody (BF647)	FC
orb1589958	14-3-3 Antibody (BF594)	FC
orb1589959	14-3-3 Antibody (BF555)	FC
orb1589960	14-3-3 Antibody (BF488)	FC
orb1589961	14-3-3 Antibody (BF405)	FC
orb1589962	14-3-3 Antibody (BF350)	FC
orb1589963	14-3-3 Antibody (Cy7)	FC
orb1589964	14-3-3 Antibody (Cy5.5)	FC
orb1589965	14-3-3 Antibody (PerCP)	FC
orb1589966	14-3-3 Antibody (Cy7)	FC
orb1589968	14-3-3 Antibody (Cy5)	FC
orb1592229	Phospho-GCN2 (Thr899) Antibody (Cy5)	FC
orb1594135	GLUT1 Antibody (BF555)	FC,IF
orb1594414	Annexin V Antibody (Cy5.5)	FC
orb1595292	LPAP Antibody (PerCP)	FC
orb1598709	Tap1 Antibody (BF405)	FC,IF
orb1600370	VEGFA Antibody (BF488)	FC,IF
orb1600770	IL-15 Antibody (BF405)	ICC,FC,IF
orb1600788	CREM Antibody (PerCP)	ICC,FC
orb1601401	PIEZO1 Antibody (BF488)	ICC,FC,IF
orb1602128	Melatonin Receptor 1A Antibody (Cy7)	FC
orb1603027	PODXL Antibody (BF555)	FC,IF
orb1606712	phospho-IKK beta (Tyr188) Antibody (Cy5)	FC
orb1608014	TGF beta Receptor I Antibody (PerCP)	FC
orb1609870	Geminin Antibody (APC/Cy5)	FC
orb1816092	3326-32-7 Antibody (FITC)	FC

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